Journal:

Article Title: Interactions between peripheral blood CD8 T lymphocytes and intestinal epithelial cells (iEC)

doi: 10.1046/j.1365-2249.1998.00566.x

Figure Lengend Snippet: T cell death induced in co-cultures with iEC, fibroblast cell lines, or iEC supernatant. (a) Peripheral blood lymphocytes (PBL) at 106 cells/ml were cultured either alone or with equal cell numbers of HT-29 or fibroblast cells (FBL) in culture media 2 (CM2). Cells were stained after 5 days with 20 μg/ml propidium iodide (PI) and 10 000 cells were acquired on a FACScan and analysed with Lysys II software. Cells were gated according to forward (FSC) and side scatter (SSC) and the percentage of PI+ viable cells in gates R1 and R2. (b) PBL at 106/ml were cultured either alone or with supernatant of 3 day HT-29 cultures. Supernatants were centrifuged at 3000 rev/min for 10 min before use. PBL were harvested at the indicated time points and the percentage of PI− cells determined. Total cell numbers (PI− and PI+) remained approximately the same for both cultures during the observation period. One of two similar experiments is shown.

Article Snippet: Human skin fibroblast cell lines from a patient with Gaucher's disease and one relative were kindly donated by Dr Clara Sa Miranda (Instituto de Genetica Medica, Porto, Portugal).

Techniques: Cell Culture, Staining, Software